Description
Ion exchange layers made from cellulose are well-suited for separating substances with exchange-active groups, leading to their use in isolating amino acids, peptides, enzymes, and nucleic acid constituents (nucleotides, nucleosides). PEI cellulose, a complex of cellulose and polyethyleneimine (not a chemical modification), acts as a relatively strong anion exchanger and requires refrigerated storage at 4°C. CEL 300 PEI is notably used in the 32P postlabeling analysis of mutagenic substances.
|
Method |
Thin layer chromatography (TLC) |
|
Brand |
POLYGRAM |
|
Phase |
CEL 300 PEI UV254 |
|
Base material |
Polyester sheets coated with cellulose |
|
Surface chemistry |
Fibrous cellulose impregnated with Polyethyleneimine (PEI) |
|
Mode |
Ion exchange (IEX) |
|
Plate size |
20x20 cm |
|
Layer thickness |
0.1 mm |
|
Layer hardness |
Standard, soft |
|
UV indicator |
Yes |
|
Support material / backing |
Polyester |
|
Particle size |
2–20 µm |
|
Particle type |
Fully porous particles (FPP) |
|
Particle shape |
Irregular |
|
Pore volume |
Not available or not applicable |
|
Binder |
Yes |
|
pH stability |
2.0–8.0 |
|
Recommended application(s) |
Ion exchange (IEX), Mutagenic substances with the 32P postlabelling procedure, Nucleic acids, Nucleosides, Nucleotides |
|
Temperature stability |
< 185 °C / < 365 °F |
|
Storage temperature |
4 °C / 39 °F |
