Column-based extraction chromatography

DNA Extraction Spin Columns:

DNA extraction is an essential step in many molecular biology applications. It allows scientists to detect and identify bacteria and viruses, as well as to study their genetic information. The purification process of DNA extraction is often done using spin columns, which are the most efficient and cost-effective way to obtain high quality DNA samples. Spin columns are easy to use, require minimal equipment, and can be used with a variety of sample types. They also provide high purity DNA samples with minimal contamination. With the help of spin columns, researchers can easily extract and purify DNA and RNA from a variety of sources such as plants, animals, bacteria, or viruses.

Molpure's SorbElut™ DNA and RNA extraction spin columns provide an innovative solution for isolating genomic or plasmid DNA from various biological samples. Our mini columns use silica gel membrane micro fiberglass filter membrane bonding chemistries, making them compatible with most commercially available kits for ease of use and versatility in research. The columns feature durable polypropylene construction and a unique silica-based glass microfiber matrix for efficient nucleic acid binding.

To use a SorbElut™ Spin Column, the DNA sample is loaded onto the column in a high salt buffer. The column is then spun down, which causes the DNA to bind to the membrane. The column is then washed with a buffer that contains a lower salt concentration. This washes away any unbound contaminants, such as proteins, salts, and RNA. The DNA is then eluted from the column using a buffer that is free of chaotropic salts. This results in a highly pure DNA sample that is ready for downstream applications, such as PCR.

Here is a brief summary of the key points:

High purity: SorbElut™ DNA or RNA Spin Columns remove contaminants such as proteins, salts, and from the sample, resulting in a highly pure DNA or RNA product.
High binding capacity or yield: Our columns can bind and purify up to 30 µg of DNA per column.
Fast and easy to use: SorbElut™ Spin Columns are easy to use and can be processed in less than 10 minutes.
Versatility: SorbElut™ Spin Columns can be used to purify DNA from a variety of sample types including blood, soil, feces, extracting cfDNA from plasma, very high copy number plasmid DNA from overnight E. coli culture bacterial lysates, high molecular weight DNA from bacterial lysate and RNA binding.